Development of transposon-mediated GAL4 gene and enhancer trap methods and production of abnormal behavioral phenotype by targeted expression of tetanus toxin light chain in zebrafish .
Asakawa, K.1 , Mizusawa, K.1 , Urasaki, A.1 , Kotani, T.1 , Nagayoshi, S.1 , Kishimoto, Y.1 , Hibi, M.2 , Kawakami, K.1
1. National Institute of Genetics, 2. RIKEN CDB
We created the Tol2 transposon constructs for GAL4 gene and enhancer trapping. A modified GAL4 gene was placed downstream of rabbit β-globin splice acceptor for gene trapping (SA-gal4) and the zebrafish hsp70 promoter for enhancer trapping (hsp-gal4). Then, we created random insertions of these Tol2 constructs in the genome. By crossing the SA-gal4 fish or the hsp-gal4 fish with the UAS-EGFP fish, we isolated 206 GAL4 lines based on unique GFP expression patterns.